SKU: 21706163309

Unione ®️ TR-FRET Human PD1/PD-L1 Binding Kit

Sale price$981.00 Regular price$1090.00
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Description

Unione ®️ TR-FRET Human PD1/PD-L1 Binding KitProduct Specification Host Human Stability & Storage 80 Background The assay kit utilizes homogeneous time resolved fluorescence (TR FRET) technology to measure the interaction between PD1 and PD L1. This method enables simple, rapid, and high throughput screening of inhibitors and antibody blockers. As shown in the figure, the interaction between PD1 and PD L1 is detected using an Eu labeled anti Tag1 antibody (TR FRET donor) and an Ac labeled anti

Product Specification


Host Human
Stability & Storage

-80℃

Background

The assay kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between PD1 and PD-L1. This method enables simple, rapid, and high-throughput screening of inhibitors and antibody blockers.

As shown in the figure, the interaction between PD1 and PD-L1 is detected using an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). The binding of PD1 and PD-L1 brings the donor and acceptor antibodies into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. Pembrolizumab, as a positive control, blocks the interaction between PD1 and PD-L1, preventing FRET signal generation. The stronger the blocking effect of the screened drug on the PD1/PD-L1 interaction, the lower the signal. The specific signal is proportional to the extent of the PD1/PD-L1 interaction. This homogeneous assay is simple to perform and requires no washing steps.

Components

Component

Concentration

100T

500T

2500T

10000T

Storage Temperature

Pembrolizumab

5μM

5μL

10μL

50μL

200μL

-80℃

Tag1-PD-L1 protein

100×

5μL

20μL

100μL

400μL

-80℃

Tag2- PD1 protein

100×

5μL

20μL

100μL

400μL

-80℃

Anti-Tag1 Eu antibody

50×

10μL

50μL

250μL

1000μL

-80℃

Anti-Tag2 Ac antibody

12.5×

40μL

200μL

1000μL

4000μL

-80℃

Detection buffer

10×

400μL

2mL

10mL

40mL

-80℃


Note: Aliquot immediately upon first thaw and store at the recommended temperature. Avoid storage after dilution and repeated freeze-thaw cycles.

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Exchange/Return Notes
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SKU: 21706163309

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Caitlyn
New York, US
★★★★★ 5
Amazing!
Size: King (Pack of 1)
Such a great pillow I’m buying another one! You can add or subtract stuffing to your liking. It’s really nice quality, quilted and soft. Best pillow ever. Super comfy and great sleep. Pretty firm with all the stuffing. Perfect the way it came with stuffing on the side. My husband loves it and now I want one.
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Reviewed in the United States on May 15, 2026
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Alli
Battle Creek, US
★★★★★ 5
Totally worth the money
Size: King (Pack of 1), Size: King (Pack of 1)
Right off the bat, the packaging is absolutely adorable and felt like I was buying a premium product. I love that this pillow is adjustable and as a side sleeper, I needed a pillow that is both firm and supportive of my neck. This hits all the boxes. We will be buying more of these for our bed!
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PublicMe
Lexington, US
★★★★★ 5
Really Happy With These Pillows!
Color: Blue, Size: Queen(2 Pack)-20'*30'
**Review: Really Happy With These Pillows!** I purchased these pillows and couldn’t be happier! First – they’re cool on *both* sides—finally! I’m not sure why that’s so rare (probably to cut costs), but these actually have cooling material on both sides, and it works really well. Second – the venting around the center helps keep them from getting hot at night. A nice touch that makes a difference for hot sleepers. However, the third feature is the best: the filling. Like many adjustable pillows, you can add or remove the fill, and these come with a small extra bag. The base amount was just right for most people. I had to remove some for my wife but added a bit more to mine. The fill itself is excellent—soft but supportive. You sink into it, but don’t hit bottom. These pillows also have a good weight to them, so they don’t feel cheap. They fluff up great and seem very well made. I followed the recommended dryer run when I first received them and had no odor issues at all. Highly recommend!
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Steve Texas
Draper, US
★★★★★ 5
Awesome Pillows
Color: Blue, Size: King(2 Pack)-20'*36'
AWESOME, they actually stay cool!!! Extra foam to firm and great instructions how to remove foam and make softer. Fantastic product
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David Hendrix
Waukegan, US
★★★★★ 4
Very fluffy and comfortable.
It only stays cool for a few minutes. It is a very comfortable pillow.
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