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Description
mIHC Dye (TSA700)Product Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 700, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.7 ★★★★★
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Product Reviews
★★★★★ 5
Exciting and insightful revelation presented in an easy-to-read way.
Format: Kindle
Matthew Robert Payne shares his personal revelations, visions, and thoughts on the end times church, the two witnesses, the 144,000 and more with a spirit of integrity and humility. I really enjoyed reading about what God had revealed to him about the two witnesses, it was radical stuff, that was not even slightly on my radar, on in my personal understanding and was exciting and interesting to read. If you want to be a part of the end times church, this part of the book is essential reading. You'll gain insight into what will happen, what you need to do to be safe and how to be used as one of God's end-time harvesters. Learn about Matthew's interpretation of who is the 144,000.This book is a must-read. It will inspire you to start living set apart for Jesus right now where you are. Read the book now!
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Reviewed in the United States on April 9, 2018
★★★★★ 4
An optimistic view of the endtimes
Format: Kindle
I like Matthew's writing style.For a subject as esoteric like Revelation, he makes it an easy read. I would have given it 5 stars if this was an audio but for some repetitious statements.
He brings to light the pivotal role of the two witnesses and the 144,000 in end-time harvest. It gives you an optimistic view of the future in keeping with the theme of the New Testament. A real blessing to the Bride waiting for the Groom. Maranatha!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 22, 2017
★★★★★ 5
End times made (more) simple.
Format: Kindle
Matthew has taken a difficult topic and expanded on it based not only on the Word, but also based on visions, prophecy from others, and revelation knowledge from the Holy Spirit. He gives new ideas and strengthens old ones. For instance, I have always thought the two witnesses in Revelation are Enoch and Elijah because God took them (they did not die) and because man is appointed once to die. This was reinforced by this book, and I believe that it is a leading by the Holy Spirit within the critical framework of the Word. Its a short read and worth your time.
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Reviewed in the United States on October 4, 2016
★★★★★ 3
Good, but ...
Format: Kindle
Not quite what I was hoping for and not nearly as informative as his other books.
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Reviewed in the United States on April 4, 2018
★★★★★ 5
The two witnesses are Elijah and Enoch
Format: Kindle
Matthew Robert Payne has the unique insight of having interviewed both Elijah and Enoch. The revelation he has received from them can be found on his YouTube channel and in his book "Great Cloud of Witnesses Speak." Those resources along with "Optimistic Visions of Revelation" give a clear picture of what the tribulation will look like. Study them to find your end time calling and how to keep your family and loved ones safe.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 8, 2017
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