SKU: 37795770240

Fixable Viability Dye 515

Sale price$94.50 Regular price$105.00
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Ships within 48 hours · Estimated delivery Jul 16 - Jul 21

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Description

Fixable Viability Dye 515Product Specification Stability & Storage 12 months from date of receipt, 20 C as supplied. Background Fixable Viability Dye 515 is excited by the Blue laser (with an excitation maximum of 488 nm) and has fluorescence emission maximum at 515 nm, Fixable Viability Dye 515 has similar emission to FITC. Fixable Viability Dye 515 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this

Product Specification


Stability & Storage

12 months from date of receipt, -20 °C as supplied.

Background

Fixable Viability Dye 515 is excited by the Blue laser (with an excitation maximum of 488 nm) and has fluorescence emission maximum at 515 nm, Fixable Viability Dye 515 has similar emission to FITC.

Fixable Viability Dye 515 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications.

Each lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.

Protocol

1.Wash cells one time in PBS buffer.

2.Add 1 µl of Fixable Viability Dye 515 Stock Solution for each 1~2 ml of cell suspension (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.

3.Resuspend cells at 1*10^7 cells/ml in Fixable Viability Dye 515, Incubate the mixture for 10-15 minutes at room temperature protected from light.

4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.

5.Resuspend the cells in Stain Buffer.

Note: This protocol applicable to mouse splenocytes Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.

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Exchange/Return Notes
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SKU: 37795770240

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@rosegoldraych
Natrona Heights, US
★★★★★ 5
High Value
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High quality, would definitely buy again!
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Reviewed in the United States on March 15, 2026
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Em Maya Ryan
Bozeman, US
★★★★★ 5
Nice Quality and Very Functional
Size: 11" x 7.5" x 0.7", Color: Marble Tray-viola Tablet Set
These look great and I love that they can be use for all sorts of things- catchall, tray, soap dish holder. They also have felt bottoms, so they protect whatever you set them. All around a nice product.
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Reviewed in the United States on December 15, 2025
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Jarrett Thomas
Lake Worth, US
★★★★★ 5
Perfect piece, an attribution to my vibe
Exactly what i wanted, fits right on the shelf I got it for, medium in size, light weight, nice quality beautiful pice, sturdy, great value for money spent
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Reviewed in the United States on January 8, 2026
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Kate45
San Leandro, US
★★★★★ 5
Nice little addition to you tabletop or bookshelf.
I liked the size and shape of the sculpture. It is small but makes a statement when stacked on top of a few books. I love it!
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Reviewed in the United States on July 2, 2023
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purple58
Carnegie, US
★★★★★ 5
Nice Addition to My Bookshelf
After Christmas my bookshelves were looking a little blah. Wanted something a little different so I thought I would try a statue. This is very nice. Looks like the picture. It's small. 6 inches. Have it sitting on top of some books. Good value for the money.
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Reviewed in the United States on February 28, 2022

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