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Description
Caspase 6 Activity Assay KitProduct Specification Usage Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12. 5, 6. 25, 3. 125 M. Set the zero tube without pNA. 2. Take 100l of each concentration and add it to a 96 well plate or 100l cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x axis, and the corresponding pNA concentration is the y axis.
Product Specification
| Usage | Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12.5, 6.25, 3.125 μM. Set the zero tube without pNA. 2. Take 100μl of each concentration and add it to a 96-well plate or 100μl cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x-axis, and the corresponding pNA concentration is the y-axis. Use Excel to make a standard curve of pNA concentration versus OD405 value. Detection of Caspase 6 enzyme activity: 1. Take out an appropriate amount of Ac-VEID-pNA (2mM) and place it on an ice bath for later use. 2. According to the 100ul reaction system: blank control group: 40μl of detection buffer, 0μl of sample to be tested, 50μl of lysate, 10μl of Ac-VEID-pNA (2mM); Sample group: 40μl of detection buffer, 50μl of sample to be tested, 0μl of lysate, 10μl3 of Ac-VEID-pNA (2mM). Add Ac-VEID-pNA (2mM) and mix well. Be careful to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight. 4. The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 6 in the sample. By comparing the standard curves obtained in the above steps, it is possible to calculate the amount of pNA catalytically produced in the sample. |
| Theory | Based on Caspase 6, it can catalyze the substrate Val Glu Ile Asp p nitroanilide (Ac VEID pNA) to produce yellow pNA (p-nitroanilide), which can be used to detect the activity of Caspase 6 by measuring absorbance. pNA has a maximum absorbance at 405 nm. Caspase activity was obtained by measuring pNA using visible light colorimetry. Suitable for detecting Caspase 6 activity in mammalian tissues and cells. |
| Experiment Reagent Instrument | Microplate reader, spectrophotometer |
| Synonym | Mch-2,caspase-6,caspase 6 |
| Description | caspase 6 Activity Detection Kit is a kit that detects caspase 6 enzyme activity or purified caspase 6 enzyme activity in cell or tissue lysates by spectrophotometry. |
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4.3 ★★★★★
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Product Reviews
★★★★★ 4
Great introduction to work management
Format: Hardcover
I am not an expert in project or work management and found this book really useful and readable. Moore does a great job of looking beyond standard project management terms and frameworks and helps re-frame project management as a strategic, not a tactical concern.
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Reviewed in the United States on October 23, 2009
★★★★★ 5
Nice update
Format: Paperback, Format: Paperback
I really like this version of a practice test. It covers pretty much all the topics I wanted to learn more about (like math related) and the fact that’s it’s current for this year is great. I didn’t want to practice with old information. It also has a well-structured approach to mastering the digital format. I feel like it properly prepares you for the actual feeling of doing the test. My favorite thing has to be the mini test. Sometimes I think I’m ready for a test but end up realising I still have a long way to practice so it’s great to have a tool that helps with that.
Just overall, its practice is very reasonable so as long as you take the time to really take advantage of this book, the SAT will be a breeze.
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Reviewed in the United States on March 11, 2025
★★★★★ 5
An exceptional resource
Format: Paperback, Format: Paperback
Vibrant Publishers did a great job on writing this SAT book. It has been researched thoroughly. Vibrant publishers has some great study lessons in here that gives great ideas for helping others with learning the SAT. The lessons are great to learn, and also the questions to study are very easy to understand and put the answers in context to answer the questions. This book I would definitely recommend to others given the chance. I hope to hear more from the author. The author made it easy to learn and study for the SAT. Vibrant publishers gave some great ideas on how to help students study for the SAT. I think there are some great ideas to helping people pass the test to go to college, and do well. I definitely give this book a 5-star rating.
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Reviewed in the United States on March 1, 2025
★★★★★ 4
Will definitely help with preparedness
Format: Paperback, Format: Paperback
When I was first planning on taking the SAT, I definitely underestimated how to be prepared. I wish I had a book like this one back then. This book has really put together a comprehensive way of getting prepared for such an important exam. Not only are there practice exams, there is also a way to estimate your actual test scores so that you can see what areas need work. Also, there are so many different variations of questions that it will really prepare your mind for the actual exam. Better than most practice test books out there.
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Reviewed in the United States on March 12, 2025
★★★★★ 5
Helpful Resource
Format: Paperback, Format: Paperback
This book is a solid option for anyone studying for the SAT. As I worked through the practice questions, I found it to be thorough and well organized, covering many sections of the test. The range of questions keeps things interesting and allows for testing knowledge across different areas. I especially appreciated how the book breaks down each section, making it easier to focus on specific topics that need improvement. I also liked that it provides detailed explanations for both correct and incorrect answers, which helped me clarify my mistakes and improve my understanding. The formatting is another plus, as it helps make practice more realistic. This is truly a helpful resource for those looking to sharpen their SAT skills and get plenty of practice!
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Reviewed in the United States on March 22, 2025