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Description
UniOne® TR-FRET Human DDB1-CRBN&GSPT1 PROTAC Binding KITProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Time ResolvedFluorescence Resonance Energy Transfer (TR FRET) technology to measure theinteraction between GSPT1 and CRBN mediated by the molecular glue CC 885 andtest compounds. This method provides a simple, rapid, and high throughputformat for detecting small molecules capable of mediating GSPT1 CRBNinteractions. As shown, the interaction betweenGSPT1 and CRBN is
Product Specification
| Host | Human |
| Stability & Storage | -80℃ |
Background
This kit utilizes Time-ResolvedFluorescence Resonance Energy Transfer (TR-FRET) technology to measure theinteraction between GSPT1 and CRBN mediated by the molecular glue CC-885 andtest compounds. This method provides a simple, rapid, and high-throughputformat for detecting small molecules capable of mediating GSPT1-CRBNinteractions.
As shown, the interaction betweenGSPT1 and CRBN is detected using an anti-Tag1 antibody labeled with Eu (TR-FRETdonor) and an anti-Tag2 antibody labeled with Ac (TR-FRET acceptor). Since themolecular glue CC-885 mediates the interaction between GSPT1 and CRBN, thedonor and acceptor antibodies are brought into proximity. Excitation of thedonor antibody triggers fluorescence resonance energy transfer (FRET) to theacceptor antibody, resulting in specific emission of a 665 nm signal. Thisspecific signal is proportional to the degree of interaction between CC-885,GSPT1, and CRBN. This homogeneous assay is simple to perform and requires nowash steps.
Components
Component |
Concentration |
100 TESTS |
500 TESTS |
2500 TESTS |
10000 TESTS |
Storage |
Tag1-DDB1/CRBN protein |
200× |
2.5μL |
13μL |
63μL |
250μL |
-80℃ |
Tag2- GSPT1 protein |
200× |
2.5μL |
13μL |
63μL |
250μL |
-80℃ |
CC-885 (4mM) |
100× |
3μL |
5μL |
25μL |
100μL |
-80℃ |
|
Anti-Tag1 Eu dye antibody |
100× |
5μL |
25μL |
125μL |
500μL |
-80℃ |
Anti-Tag2 Ac antibody |
100× |
5μL |
25μL |
125μL |
500μL |
-80℃ |
Detection buffer |
1× |
2ml |
10mL |
50mL |
200mL |
4℃ |
*Avoid repeated freeze-thaw cycles of reagents. Recommend aliquoting after first thaw and storing according to conditions specified for a shelf life of one year.
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
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