SKU: 80149533362

CKS1 GST Tag Protein, Human

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Description

CKS1 GST Tag Protein, HumanProduct Specification Species Human Synonyms CKS 1 Accession P61024 Amino Acid Sequence M1 K79 with GST Tag at the N Terminus Expression System E. coli Molecular Weight 30 35kDa (Reducing) Purity 95% by SDS PAGE & > 90% by HPLC Conjugation Unconjugated Tag GST Tag Physical Appearance Lyophilized powder Storage Buffer PBS, PH7. 4, 5% trehalose Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water after rapid

Product Specification


Species Human
Synonyms CKS-1
Accession P61024
Amino Acid Sequence

M1-K79 with GST Tag at the N-Terminus
Expression System E.coli
Molecular Weight

30-35kDa (Reducing)
Purity ＞95% by SDS-PAGE & > 90% by HPLC
Conjugation Unconjugated
Tag GST Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Ganoth D, Bornstein G, Ko TK, Larsen B, Tyers M, Pagano M, Hershko A. The cell-cycle regulatory protein Cks1 is required for SCF(Skp2)-mediated ubiquitinylation of p27. Nat Cell Biol. 2001 Mar;3(3):321-4.
2.Sitry D, Seeliger MA, Ko TK, Ganoth D, Breward SE, Itzhaki LS, Pagano M, Hershko A. Three different binding sites of Cks1 are required for p27-ubiquitin ligation. J Biol Chem. 2002 Nov 1;277(44):42233-40.

Background

CKS1 is a 9-kDa accessory protein that binds conserved “CKS” pockets on CDK1 and CDK2. By docking the SCF Skp2 E3 ligase to CDK–cyclin complexes, it licenses ubiquitination of the CDK inhibitor p27Kip1, thereby driving G1→S progression. Elevated CKS1 is frequent in solid tumours and is an independent marker of metastasis and poor prognosis.

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SKU: 80149533362

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4.3 ★★★★★
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J
John Long
Massapequa, US
★★★★★ 4
Great intro to for those who feel drawn to apologetics
Format: Kindle
James Sire has been a wonderful companion to many Christians who are called to use their intellect. I have been a fan of his ever since his book Scripture Twisting. Sire provides a great service to the Christian community first by identifying the many ways in which we engage in defense of the faith. We tend to think of great speakers in front of skeptical audiences, but there are many other ways in which apologetics is useful and necessary, such as when we are speaking to friends. Any time we are responding to their objections to Christianity, we can do so in a winsome and knowledgeable manner, even if we aren't an intellectual giant like Sire. Second, Sire identifies that types of personality characteristics and character traits necessary to pursue apologetics as a vocation. Of course, the primary characteristic is one of pursuing God. Included with this is some discussion about how we might recognize a calling in defense of the faith. Many pastors want to equip us in ministry, but Sire's discussion actually gets down to helping us recognize if this is our calling. Sire provides some pointers to great books on the major issues we might have to deal with. It's always good to have a book of great sources I also like the many examples he provides in his book about encounters with people, including mistakes he has made. I hope this will have a wide audience and encourage others to consider this calling.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 14, 2018
B
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BG
Draper, US
★★★★★ 1
Weak
Format: Paperback
I very weak primer on apologetics. The author is really not convinced himself; so, it is difficult for him to present convincing arguments.
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Reviewed in the United States on February 9, 2013
M
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Marlene
Port Orchard, US
★★★★★ 5
Helpful for newcomer
Format: Hardcover
I find this book helpful as a new MSK provider. A video component addition would be eben better
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 12, 2023
S
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SportsMD
Pawtucket, US
★★★★★ 5
Wonderful educational textbook
Format: Hardcover
Wonderful educational textbook. Gets to the "meat". Short easy to understand site specific references that are good for the beginner or the pro.
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Reviewed in the United States on October 6, 2016
K
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Kindle Customer
Battle Creek, US
★★★★★ 4
Nice work. Thanks a lot
Format: Kindle
"From practice into practice". Nice work. Thanks a lot.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 24, 2014

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