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Ships within 48 hours · Estimated delivery Jul 16 - Jul 21
For Your Every Summer RSVP, with Code: SUMMER15
Description
Poly(A) Polymerase Tailing KitProduct Specification Stability & Storage Store at 25 ~ 15 for 2 years Reference Components UA070116 50 Rxns UA070116 250 Rxns E. coli Poly(A) Polymerase (5 U l) 100 l 5 100 l 10 X PAP Reaction Buffer 300 l 5 300 l 10 mM ATP 300 l 5 300 l RNase Free dH2O 1 ml 5 1 ml Protocol 1. Prepare the following reaction system on ice according to the table below: Component Volume Final Concentration 10 X PAP Reaction Buffer 5 l 1 X RNase Inhibitor (40U l) 1. 25 l
Product Specification
| Stability & Storage | Store at -25 ~ -15℃ for 2 years |
| Reference |
Components
组分 |
UA070116-50 Rxns |
UA070116-250 Rxns |
|
E. coli Poly(A) Polymerase (5 U/µl) |
100 μl |
5 × 100 μl |
10 X PAP Reaction Buffer |
300 μl |
5 × 300 μl |
10 mM ATP |
300 μl |
5 × 300 μl |
RNase Free dH2O |
1 ml |
5 × 1 ml |
Protocol
1. Prepare the following reaction system on ice according to the table below:
Component |
Volume |
Final Concentration |
10 X PAP Reaction Buffer |
5 μl |
1 X |
RNase Inhibitor (40U/μl) |
1.25 μl |
1 U/μl (Optional) |
|
E.coli Poly(A) Polymerase (5 U/μl) |
2 μl |
0.2 U/μl |
10 mM ATP |
5 μl |
1 mM |
RNase Free dH2O |
Up to 50 μl |
- |
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