SKU: 97186239198

Furin/PCSK3 His Tag protein, Mouse

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Description

Furin/PCSK3 His Tag protein, MouseProduct Specification Species Mouse Synonyms FUR,PCSK3,SPC1,PACE Accession P23188 Amino Acid Sequence Gln25 Glu714 with His Tag at the C Terminus Expression System HEK293 Molecular Weight 72 95kDa (Reducing) Purity 85% by SDS PAGE Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized powder Storage Buffer PBS, PH7. 4, 5% trehalose Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water after rapid

Product Specification


Species Mouse
Synonyms FUR,PCSK3,SPC1,PACE
Accession P23188
Amino Acid Sequence

Gln25-Glu714 with His Tag at the C-Terminus

Expression System HEK293
Molecular Weight

72-95kDa (Reducing)

Purity >85% by SDS-PAGE
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose

Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· Please do not repeated freeze-thaw cycles.

Reference

1.Fitzgerald K. Furin Protease: From SARS CoV-2 to Anthrax, Diabetes, and Hypertension. Perm J. 2020;24:20.187.
2.Dahms SO, Hardes K, Becker GL, Steinmetzer T, Brandstetter H, Than ME. X-ray structures of human furin in complex with competitive inhibitors. ACS Chem Biol. 2014 May 16;9(5):1113-8.

Background

Furin, also known as Paired Basic Amino Acid Cleaving Enzyme (PACE), is a key member of the subtilisin-like proprotein convertase family. Functioning as a calcium-dependent serine protease, its structure includes an N-terminal signal peptide, a prodomain, a catalytic domain, a P-domain responsible for maintaining enzymatic activity and calcium/pH sensitivity, a transmembrane region, and a cytoplasmic tail. Primarily localized within the Golgi apparatus/endosomal system, it activates a wide range of proprotein substrates—such as growth factors, receptors, extracellular matrix proteins, and pathogen proteins (e.g., envelope proteins of HIV and influenza virus)—by specifically cleaving after the Arg-X-(Arg/Lys)-Arg sequence motif. It plays a critical role in embryogenesis, peripheral immune tolerance, and cellular homeostasis. Dysregulation of its function is closely linked to tumor progression, neurodegenerative diseases, and various pathogen infections, making it a significant therapeutic target in clinical applications.

Protocol

Assay protocol
Principle: Measured by its ability to cleave the fluorogenic peptide substrate, Pyr-Arg-Thr-Lys-Arg-AMC.
Materials
Assay buffer: 25 mM Tris, 1 mM CaCl2, pH 9.0
Furin/PCSK3 His Tag protein, Mouse (UA011277)
Substrate: Pyr-Arg-Thr-Lys-Arg-AMC (abs45133051)
96 ELISA Removable Plate, Black, High binding (GENEVER, Catalog # GMO2-96H)
Plate Reader (PerkinElmer, excitation 380 nm and emission 460 nm)
Produce
Dilute Mouse Furin Protein to 2 µg/mL/4 µg/mL/8 µg/mL in Assay Buffer.
Dilute Substrate to 100 µM in Assay Buffer.
Load into a black well plate 50 µL of 2 µg/mL/4 µg/mL/8 µg/mL of Mouse Furin Protein, and start the reaction by adding 50 µL of 100 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 100 µM Substrate.
Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in kinetic mode for 10 minutes.
Calculate specific activity:
*Adjusted for Substrate Blank
**Derived using calibration standard 7-amino-4-Methyl Coumarin
Conversion Factor (pmol/RFU)
Produce
Buffer: Assay buffer
Calibration standard: standard 7-amino-4-Methyl Coumarin (Enzo, Catalog # BML-KI107-0001).
Add 100 µL Dilute Calibration standard, Standard curve 1500/750/375/188/94/47/24/12/6/3 pmol per well or blank (Assay buffer) per well.
Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in endpoint mode.
Linear Regression of 7-amino-4-Methyl Coumarin (pmol) (y)—RFU-Blank(x).
Note
Storage Conditions: After reconstitution, aliquot and store at -80°C. Please do not repeated freeze-thaw cycles.

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SKU: 97186239198

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Stephen H. Gorman
Pawtucket, US
★★★★★ 5
Huron
Scent: Hair Styler (1 Pack)
Great product
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 30, 2025
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Verified Purchase
Timothy
Omaha, US
★★★★★ 3
Not bad but read my full review if you are experimenting with different brands
Scent: Hair Styler (1 Pack)
So I am middle of the road on this product. First, I love the products from this brand and use some of their other products quite regularly. I was on a search for an alternative to the Crew Forming Cream (which I have used for maybe 20 years) and decided to purchase this product by Huron as well as the much cheaper medium hold styling cream by Cremo. All three are great products but you will need to decide for yourself how much hold you need as I have discovered that each are quite different and really shouldn't be compared to one another. I will say that if I had to put them all into two categories then Crew is heavy hold and Huron and Cremo is medium / light hold. I must admit that I was skeptical that the cheaper of the three, Cremo, would win me over but it has. For the record it has a very subtle scent (hardly noticable), just enough hold and does not leave you feeling like you have product in your hair. This is similar to Huron as well (although Huron is a very creamy in texture and you can tell its a higher quality product) but at the end of the day all that matters is great hold from a product that is affordable and does not leave a sticky texture in your hair. Cremo wins hands down. As for Crew, well it was a good ride but too expensive and way too heavy...time to move on.
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Reviewed in the United States on March 1, 2025
C
Verified Purchase
coronad0
Port Orchard, US
★★★★★ 5
So lightweight. Perfect for extra thick hair.
Scent: Hair Styler (1 Pack)
Its almost mousse like in how light weight it is and smells fantastic. I wanted something just above light weight hold and not weigh my thick hair down and this does a great job. A little pricey but SO much better than the usual store brands out there. And my wife is totally in love with how great it smells when I put it on, so bonus!
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Reviewed in the United States on May 22, 2024
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Verified Purchase
Jason Kearns
Boise, US
★★★★★ 5
Smells good and doesn’t feel greasy
Scent: Hair Styler (1 Pack)
Product didn’t leave a greasy feeling to the hands or the hair. Kept hold all day for my hair style but I knew it was light hold going in. It has a great scent
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Reviewed in the United States on October 15, 2024
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Matthew M
Bozeman, US
★★★★★ 5
No texture issues
Scent: Hair Styler (1 Pack)
I’ve never been one to like hair product because I don’t like how they make my hair feel “crunchy” or overly stiff. However, this kind provides a nice, loose hold that doesn’t make my hair feel weird. If you can spare the extra few dollars and don’t need a strong hold, I would recommend this!
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Reviewed in the United States on May 15, 2025

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