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Description
MTS Cell Proliferation Assay KitProduct Specification Usage Experimental operation: 1. MTS working fluid preparation: Mix MTS Solution and PMS Solution at a ratio of 20: 1 to obtain the MTS working solution. If the prepared MTS working solution needs to be stored, it should be stored at 20 in the dark from light. At this time, it is recommended to dispense appropriate amounts to avoid repeated freezing and thawing. Note: Both MTS Solution and PMS Solution are easy to decompose when
Product Specification
| Usage |
Experimental operation: 1. MTS working fluid preparation: Mix MTS Solution and PMS Solution at a ratio of 20: 1 to obtain the MTS working solution. If the prepared MTS working solution needs to be stored, it should be stored at-20 ℃ in the dark from light. At this time, it is recommended to dispense appropriate amounts to avoid repeated freezing and thawing. Note: Both MTS Solution and PMS Solution are easy to decompose when exposed to light, so they need to be protected from light during preparation and use (can be wrapped in tin foil). If you want to store this kit at 4 °C for frequent use, the MTS working solution should be prepared before each test. The prepared unused MTS working solution needs to be stored at-20 °C protected from light. 2. Cell culture: The cells are seeded in a suitable container such as a well plate. For 96-well plates, it is recommended to add 100uL of medium per well, and it is recommended to inoculate 5-100 × 10 per well3Cells. The cells of the experimental group can be treated with the drug of interest for a period of time according to the experimental design, at which time it is necessary to set up a blank group (containing medium only) and a control group (containing medium, cells and drug solvent). Note: The specific number of cells seeded per well can be adjusted according to the specific cell type. 3. MTS incubation: 20 uL of MTS working solution was added to each well and then incubated in a cell incubator for 1-4 h. Note: The MTS incubation time can be adjusted for specific experiments. 4. Detection: The absorbance (A) was measured at 490 nm with a microplate reader. If a 490 nm filter is not available, optionally use a 450-540 nm filter. 5. Data analysis: The cell survival rate is calculated as follows Cell viability (%) = [(AExperimental group-ABlank group)/(AControl group-ABlank group)] ×100 |
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| Beilstein | 0 | |||||||||
| Description | MTS Cell Proliferation Assay Kit (MTS Cell Proliferation Assay Kit) is a common kit for detecting cell proliferation and toxicity. MTS is a novel tetrazolium salt, which can be reduced by succinate dehydrogenase in mitochondria of living cells to the water-soluble product Formazan. Formazan is specifically absorbed at 490 nm, so cell proliferation can be reflected by measuring the absorbance at 490 nm. The faster the cell proliferation, the higher the absorbance value. Compared with the common MTT kit, this kit does not need to remove the original culture medium, nor does it need to use another solvent to dissolve formazan, which is more convenient to use, and can avoid experimental errors caused by taking away part of formazan when removing the culture medium. This kit is very flexible to use, and incubation can be continued after detection to obtain better color development results. In addition, the kit has the advantages of wide linear range, high sensitivity and safe use. Product Components:
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| PubChem CID | 0 | |||||||||
| General Notes | 1. When the cells are cultured for a long time, the edge wells of the 96-well plate are prone to liquid evaporation. It is recommended to discard the circle around the 96-well plate and add sterile water, culture medium or PBS instead; At the same time, the 96-well plate can be placed in the incubator close to the water source. 2. MTS Solution and PMS Solutin will solidify at low temperatures. Before use, please return to room temperature or 37 °C water bath for about 10 minutes until completely melted before use. 3. MTS Solution and PMS Solution are sensitive to light. If stored at 4 °C, the color of the solution may change slightly. This change may increase the background absorbance value, but it will not affect the final experimental results. 4. If it cannot be detected in time, you can add 25uL of 10% SDS to each well after the incubation in Step 3 to terminate the reaction. The SDS-treated well plate can be placed Store in an incubator protected from light for no more than 18 hours. 5. For your safety and health, please wear a lab coat and disposable gloves. |
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| Storage Temp. | Store the kit at -20°C away from light and moisture, stable for 1 year. For frequent use, the kit can be stored at 4°C away from light and moisture, stable for 6 weeks. |
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4.3 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 5
Excellent Quality, Great Value, Great Look. Highly Recommend
Very well-made, there are some minor imperfections in the cuff sewing that aren't really noticeable when on. Liner is quality, color is spot on, and it fits true to size. If you're in the market for a toddler blazer and pants, you can't go wrong with this one in terms of quality, price, and the cut is perfect for a very polished look.
There were a few loose strings to be clipped, but everything was correctly sewn and well. None of them affected the seam strength. The pants have a button and elastic on the waistband to make adjusting the waist size easier.
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Reviewed in the United States on November 14, 2025
★★★★★ 5
Great boys slim fit suit for weddings, church, or special events
Color: Medium Grey, Size: 12
Great kids suit. Comes with a jacket, pants, and tie. Does not have a shirt. Fits as expected. Classic cut on the lapels. Great quality materials. Well made pants and jacket. The color is consistent without any fade or dark spots that can happen with lower quality fabrics. Looks great on. My son loves it. Says it is not restrictive, he can move easily in it. Highly recommend for church, special events, weddings, etc.
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Reviewed in the United States on October 22, 2025
★★★★★ 5
Nice high-quality, light weight suit for a tween!
Color: Navy Blue, Size: 10
This is such an attractive suit for a tween boy! My son loves dressing up, and this is his new favorite suit. He really likes the double-breasted style and the light weight of the fabric so that it isn't too hot. The tie that came with it gives major Harry Potter vibes which we weren't expecting, but he has lots of other ties that he also enjoys pairing with it. The size seems right, and it fits him well. He says it is very comfortable. I'm happy with the quality and the navy color.
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Reviewed in the United States on October 14, 2025
★★★★★ 5
Stylish and great value!
Color: Medium Grey, Size: 14
This grey suit was a great find for a school ceremony and will definitely be used again. The neutral color makes it easy to pair with different shirts and ties, and it has a modern, clean-cut look. The jacket and pants fit nicely after a quick press, and the quality was better than I expected for the price. It lost a star only because the waist on the pants needed a slight adjustment, but overall, we’re very happy with it!
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Reviewed in the United States on October 10, 2025
★★★★★ 3
Runs big, and fabric may not be same color as picture
Color: Medium Grey, Size: 16, Color: Medium Grey, Size: 16
This suit was a good find for us, as we usually dress up for church, and my 13 yo son often wears suits. He normally wears a size 16, so that’s what we ordered, and in dark charcoal gray. The suit we got was a light, heathered gray that didn’t look much like the one in the picture. It was very wrinkly and thin, and a little scratchy. Also, the sleeves were way too long and the torso cut of the jacket was pretty wide. The pants were very large in the waist, but fit him decently in the length (he’s 5’4” and weighs 90 lbs). Overall the suit looked like it was drowning him, so I would order a size down unless you have a larger child/teen. Because of the ill fit, I haven’t had the chance to see what the suit looks like on him as it’s supposed to, but I guess I’ll return it or wait a year or two until he fills it out a bit more. Not overly impressed with the quality, so I’m not sure I’d pay $55 for this one again.
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Reviewed in the United States on October 20, 2025
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