SKU: 29414995514

UniOne® TR-FRET Human Epigeneous Bromodomain Binding Kit

$981.00 $1090.00
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Description

UniOne® TR-FRET Human Epigeneous Bromodomain Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Homogeneous Time Resolved Fluorescence(TR FRET) technology to measure the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1 21) peptide. This method enables simple and rapidhigh throughput screening of inhibitors and blocking antibodies. As illustrated, the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1 21) peptide is detected using a Eu labeled Anti

Product Specification


Host Human
Stability & Storage

-80℃

Background

This kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1-21) peptide. This method enables simple and rapidhigh-throughput screening of inhibitors and blocking antibodies.

As illustrated, the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1-21) peptide is detected using a Eu-labeled Anti-Tag1antibody (TR-FRET donor) and an Acceptor-labeled Anti-Tag2 antibody (TR-FRETacceptor). The binding of BRD4 to the [LYS(5,8,12,16)AC] H4(1-21) peptidebrings the donor and acceptor antibodies into close proximity. Excitation ofthe donor antibody then triggers Fluorescence Resonance Energy Transfer (FRET)to the acceptor antibody, resulting in a specific emission signal at 665 nm.The intensity of this specific signal is proportional to the degree ofinteraction between BRD4 and the [LYS(5,8,12,16)AC] H4(1-21) peptide. Thishomogeneous assay is simple to perform and requires no washing steps.



Components

Table 1. Kit Components and Storage Conditions

Component

Concentration

100 TESTS

500 TESTS

2500 TESTS

10000 TESTS

Storage Temp.

(+)-JQ-1

300μM

3μL

10μL

50μL

200μL

-80℃

Tag1-BRD4 protein

100×

4μL

20μL

100μL

400μL

-80℃

Tag2- peptide

100×

4μL

20μL

100μL

400μL

-80℃

Anti-Tag1 Eu dye antibody

50×

10μL

50μL

250μL

1000μL

-80℃

Anti-Tag2 Ac antibody

12.5×

40μL

200μL

1000μL

4000μL

-80℃

Detection buffer

10×

400μL

2mL

10mL

40mL

-80℃

* Avoid repeated freeze-thaw cycles of reagents. Aliquot upon first thaw and store according to the specified conditions. Shelf life is one year.

Shipping Notes
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Exchange/Return Notes
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SKU: 29414995514

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The Pacific Northwesterners
Natrona Heights, US
★★★★★ 4
Comfortable and Easy to Wear Daily
Size: 2 Little Kid, Color: Navy/Blue
My daughter has been wearing these navy/blue shoes since the weather has been less rainy and more sunny. They are great for outdoor play, especially on days when it's warm outside. The knit mesh upper keeps her feet from overheating, and the cushioned insole feels supportive without being stiff. At size 2, the fit is accurate and the width runs a bit roomy, which gave her some growing room without feeling loose. The slip-on design with elastic laces and pull tabs is a real plus. She can get them on and off without any help, which she loves. The shoes are light enough that she barely notices them during long active days. My one small complaint is that the sole feels more like firm foam than rubber, so they can be a little slippery on smooth indoor floors. For the price, these deliver solid everyday value. They look good, hold up well to regular use, and my daughter reaches for them first most mornings. Pros: - Breathable mesh keeps feet cool - Easy slip-on design with elastic laces and pull tabs - Lightweight and comfortable for all-day wear - Accurate sizing with a roomy width - Durable for the price Cons: - Sole can be slippery on hard smooth surfaces - Limited outsole grip compared to rubber-soled shoes
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 1, 2026
D
DR
Chelsea, US
★★★★★ 5
Great kids' shoe — easy on, comfy all day
Size: 3 Little Kid, Color: Pink/Lavender
My kid has been wearing these for a few weeks now and I'm genuinely impressed with how well they've held up. The knit mesh upper keeps feet cool and comfortable during long days at school and outdoor play, and the cushioned EVA midsole does a great job absorbing impact. What really won me over is the slip-on design with elasticated laces and dual pull tabs — my child can put them on and take them off completely independently, which is a huge win. The fit is snug but not tight, and they run a bit roomy in width, which is great for kids with wider feet or those who need a little growing room. At $32, the quality feels solid for the price. Lightweight, breathable, and easy to wear — these check all the boxes for an everyday kids' shoe.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 23, 2026
M
Melissa
Charlottesville, US
★★★★★ 5
Cute, Comfortable, Light-Weight Shoes, Perfect for School
Size: 11 Little Kid, Color: Pink/Lavender, Size: 11 Little Kid, Color: Pink/Lavender
These have turned out to be great everyday shoes for my 5 year old. She normally wears a size 10, but since that size wasn’t available, I ordered the 11 expecting her to grow into them. I was pleasantly surprised that they already fit her pretty well. There’s still a little extra room in the toe area, but the shoes fit securely around her ankles so they don’t feel loose or slip off while walking or playing. She says they’re very comfortable and loves how easy they are to slip on by herself. The lightweight design is nice for everyday wear, and they’ve held up well so far despite being worn almost daily. They've become her favorite shoes to wear! Also the pink/lavender color is really pretty in person. Overall, I’m very happy with them and would order again.
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Reviewed in the United States on May 13, 2026
A
Amazon customer
Lexington, US
★★★★★ 5
Great shoe well made
Size: 13 Little Kid, Color: Pink/Lavender, Size: 13 Little Kid, Color: Pink/Lavender
Really good shoes at a fair price. My daughter loves the pink and purple gradient colors. Really beautiful. Shoes have proven themselves very durable. She's been wearing these a few times a week for the last couple months and they still look new aside from some dirt stains. Fit perfectly. Very comfortable. This is always her first pick in the morning when she's getting dressed. Good breathability you can wear these in hot summer weather without sweaty feet. Versatile shoe looks good enough to wear out and about or exercise in.
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Reviewed in the United States on May 16, 2026
K
Verified Purchase
Kevin Keegan
San Leandro, US
★★★★★ 3
Runs large
Size: 7 Big Kid, Color: White
Runs very large.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 5, 2026

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